Composite
Part:BBa_K2309003:Design
Designed by: Haohui Fang Group: iGEM17_XJTLU-CHINA (2017-09-27)
AIP-induced expression system optimized for L.lactis(RBS+AgrC+RBS+AgrA+P2)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1267
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The whole part has been optimized to be compatible for expression in lactococcus lactis.
Source
The prototype of the sequence was from Staphylococcus aureus genomic DNA. Meanwhile, the part was extracted from a prior part numbered BBa_I746100.