Composite

Part:BBa_K2309003:Design

Designed by: Haohui Fang   Group: iGEM17_XJTLU-CHINA   (2017-09-27)


AIP-induced expression system optimized for L.lactis(RBS+AgrC+RBS+AgrA+P2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1267
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The whole part has been optimized to be compatible for expression in lactococcus lactis.


Source

The prototype of the sequence was from Staphylococcus aureus genomic DNA. Meanwhile, the part was extracted from a prior part numbered BBa_I746100.

References